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These arachnids usually defend themselves only when provoked or threatened. Hobo spiders are extremely protective of their egg sacs and will bite if they perceive a threat to their young. Oftentimes, humans do not realize that they are encroaching upon a hobo spider. This often occurs when a spider is residing in dark areas.
An initial study of the hobo spider venom reported their venom to be a medical threat that would produce necrotic lesions. Subsequent research has dispelled this, and the spider is no longer considered a medically threatening spider.