Rosa Klebb played by Lotte Lenya is the fifth Villain of From Russia With Love. Rosa Klebb is given the task of executing Kronsteen's plan to steal a Russian Lektor and execute Bond. Klebb recruits Tatiana Romanova, who believes she is still working for SMERSH, and that she is doing a mission for her country.

Klebb also recruits Donald ‘Red’ Grant for the role of executing 007.

After escaping Morzeny's Poison Tipped Spiked Shoe, Ernst Stavro Blofeld sends Klebb out to personally recover the Lektor and kill Bond. Klebb poses as a hotel maid in Bond's room where she tries to subtly sneak out with the Lektor. When this fails she holds Bond at gunpoint. As Klebb and Tatiana start to leave the room with the Lektor, at the last moment Tatiana distracts Klebb, dropping her gun. Bond and Klebb fight, with Bond trying to keep her poison tipped shoe at a distance. Tatiana recovers Bonds gun and after a brief moment of hesitation of who to shoot, she kills Klebb.