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Pamela Lillian Isley was an attractive and talented botanist who lived in Seattle. She became seduced by a criminal named Marc LeGrande, who needed her to assist him in stealing an Egyptian artifact with an ancient herb inside it. After the crime was committed, Legrande decided that Isley knew too much to be allowed to live, so he poisoned her with the herb inside the artifact.
Fortunately for Isley, she survived and became immune to all manner of toxins and diseases. She realized that she was completely unaffected by certain plant-based hypnotic toxins that she could use to control others. Calling herself Poison Ivy, she began wearing a revealing green plant-like costume and turned to a life of crime, combining her natural allure with hypnotic plant toxins in order to manipulate people (particularly men) into doing her bidding. After moving to Gotham City, she inevitably came into conflict with Gotham's greatest crime-fighter, Batman. Ivy nearly seduced Batman into becoming her partner-in-crime during their first encounter, and went on to become one of his most dangerous foes.